Sensitive immune dot blot test for diagnosis of Chlamydia trachomatis infection

Sensitive immune dot blot test for diagnosis of Chlamydia trachomatis infection.

The sensitivity and specificity of an immune dot blot test (IDBT), which relies on a 125I-labeled genus-specific monoclonal antibody to detect the Chlamydia lipopolysaccharide (LPS) antigen, were improved by pretreatment of specimens with proteinase K. This enzyme destroys protein A and therefore eliminates false-positive reactions caused by the presence of Staphylococcus aureus. Proteinase K t...

Immune dot blot technique for diagnosing infection with Chlamydia trachomatis.

An immune dot blot test (IDBT) to detect the genus specific lipopolysaccharide chlamydial antigen is described, in which the antigen is trapped on nitrocellulose membrane and then detected with a monoclonal antibody labelled with 125iodine. A preliminary comparison of 270 specimens obtained from the endocervix or male urethra showed that the IDBT was more sensitive (sensitivity 90%) than a comm...

Diagnosis of Chlamydia trachomatis Infection

Diagnosis and treatment of Chlamydia trachomatis infection.

Chlamydia trachomatis infection most commonly affects the urogenital tract. In men, the infection usually is symptomatic, with dysuria and a discharge from the penis. Untreated chlamydial infection in men can spread to the epididymis. Most women with chlamydial infection have minimal or no symptoms, but some develop pelvic inflammatory disease. Chlamydial infection in newborns can cause ophthal...

Chlamydia trachomatis Infection in HIV-Infected Women: Need for Screening by a Sensitive and Specific Test

Reproductive tract infection (RTIs)/sexually transmitted infections (STIs) are recognized as a major public health problem, particularly due to their relationship with HIV infection. Early detection and treatment of Chlamydia trachomatis infection (CTI) among HIV-infected and HIV-uninfected women may impact heterosexual HIV transmission. A total of 120 participants were enrolled: 30 HIV seropos...